Influence of pH on adsorption of human immunoglobulin gamma, human serum albumin and horse myoglobin by commercial chromatographic materials designed for downstream processing of monoclonal antibodies
Department of Chemical and Biochemical Engineering, Institute of Chemical and Environmental Engineering, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37 Bratislava, Slovakia
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Abstract: Static adsorption capacity was measured for three different proteins: human immunoglobulin gamma, human serum albumin and horse skeletal muscle myoglobin. Eight commercial chromatographic materials were tested: FractoGel EMD SE HiCap (M), SP Sepharose Fast Flow, S Ceramic HyperD F, MabSelect, rProtein A Sepharose Fast Flow, Poros 50A High Capacity, ProSep –vA High Capacity and MEP HyperCel. The adsorption capacity was measured at pH values of 4.0, 5.0, 6.0, 7.0 and 8.0.
Keywords: adsorption capacity, chromatography, horse myoglobin, human immunoglobulin gamma, human serum albumin
Full paper in Portable Document Format: acs_0008.pdf
Acta Chimica Slovaca, Vol. 1, No. 1, 2008, pp. 85—94