Biocompatibility and cytotoxicity of bioglass-ceramic composite with various P2O5 content in Li2O-SiO2-CaO-CaF2-P2O5 system on fibroblast cell lines
Institute of Biochemistry, Nutrition and Health Protection, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, SK-81237 Bratislava, Slovakia
a Institute of Polymer Materials, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, SK-81237 Bratislava, Slovakia
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Abstract: Synthetically prepared biomaterials that are used in clinical practise as alternatives of damaged, diseased or undeveloped parts of skeleton as well as missing parts of body for correction of inherited or pathological deformities or for traumatic injuries are the most often used materials. To improve the surface properties of biomaterials for cell adhesion and colonization, the bioglass with various content of P2O5 in oxide system Li2O-SiO2-CaO-CaF2-P2O5 was prepared. In this work the biocompatibility and cytotoxicity of bioglass with 0%, 10%, 12% and 14% content of P2O5 were evaluated on mouse fibroblast NIH-3T3 cells and human fibroblast cells VH10 and B-HNF-1. The dishes without bioglass presence were used as negative control. We found that all used cell lines were sensitive to bioglass and all used methods have indicated slight cytotoxicity. The inhibition of cell proliferation was concentration- and time- dependent. The difference was observed among bioglass with various content of P2O5. The microscopic observations shown that control cells grew on the surface of the cultivation flask. The vast majority of them were scattered and exhibited a typical fibroblast morphology with an elongated and polygonal shape. All fibroblast cells growing on the surface of bioglass were homogeneously distributed on the substrate with good colonization. On the basis of the obtained results it can be concluded that bioglass with various content of P2O5 in oxide system Li2O-SiO2-CaO-CaF2-P2O5 showed a slight cytotoxicity and very good biocompatibility.
Keywords: bioglass-ceramic, Li2O-SiO2-CaO-CaF2-P2O5 system, fibroblast cell lines, direct contact, biocompatibility, cytotoxicity
Full paper in Portable Document Format: acs_0077.pdf
Acta Chimica Slovaca, Vol. 4, No. 1, 2011, pp. 15—30