Acta Chimica Slovaca (ACS) publishes papers on fundamental and applied aspects of chemistry, biochemistry, chemical technology, chemical engineering and process control, biotechnology and food technology. Welcome are also topics which include chemical aspects of materials, physical chemistry and chemical physics, analytical chemistry, macromolecular chemistry and biomedical engineering.

Tests of lipid peroxidation products using the DNA based electrochemical biosensor

Adriana Ferancová *, Katarína Beníková, Júlia Galandová, Ľudmila Sirotová a, Ján Labuda

Institute of Analytical Chemistry, Slovak University of Technology, Radlinského 9, 812 37 Bratislava, Slovakia
a Food Research Institute, Biocentrum, Kostolná 7, 900 01 Modra, Slovakia

E-mail: *,

Abstract: In this work screen-printed carbon electrodes (SPCE) were modified with different nanocomposites: chitosan - multi-walled carbon nanotubes (CHIT-MWNT/SPCE), chitosan - single-walled carbon nanotubes (CHIT-SWNT/SPCE) and chitosan - carboxylated single-walled carbon nanotubes (CHIT-SWNT-COOH/SPCE) and further modified with DNA. Cyclic voltammetry and electrochemical impedance spectroscopy were used to characterize basic electrochemical properties of the prepared sensors and biosensors. For the lipid peroxidation tests, DNA was first incubated with malondialdehyde (MDA) and oxidized edible oils, then isolated and put onto the surface of CHIT-SWNT-COOH/SPCE. Adducts formation of MDA and lipid peroxidation products with DNA was found. These adducts inhibit the diffusion of the electrochemical probe [Fe(CN)6]3−/4− to the electrode surface and, thus, changes in the relative charge-tranfer resistance were observed. These changes are in good agreement with the amount of lipid peroxidation products determined by the peroxide and TBARS values.

Keywords: DNA biosensor, lipid peroxidation, screen-printed carbon electrode, chitosan, carbon nanotubes, edible oils

Full paper in Portable Document Format: acs_0006.pdf

Acta Chimica Slovaca, Vol. 1, No. 1, 2008, pp. 58—71