Flow-through electrochemical system with the DNA-based biosensor for the evaluation of deep DNA damage by chemicals and effect of antioxidants
Institute of Analytical Chemistry, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Radlinského 9, 81237 Bratislava, Slovakia
Abstract: In this work we present an electrochemical DNA biosensor arranged in a flow-through cell which was used for the evaluation of DNA damage. The DNA-based biosensor is created using a layer double stranded calf thymus DNA deposited on the surface of a screen-printed carbon electrode. The DNA damage by the Fenton reaction agents is detected by cyclic voltammetry parameters of the [Fe(CN)6]3–/4- complex used as a redox indicator in the phosphate buffer solution. Electrochemical impedance spectroscopy is used for the verification of changes of the DNA layer. The biosensor was also used for the detection of antioxidative effects of standard chemicals and tea extracts.
Keywords: DNA damage, biosensor, flow-through electrochemical system, screen-printed carbon electrode
Full paper in Portable Document Format: acs_0052.pdf
Acta Chimica Slovaca, Vol. 2, No. 2, 2009, pp. 129—138