Acta Chimica Slovaca (ACS) publishes papers on fundamental and applied aspects of chemistry, biochemistry, chemical technology, chemical engineering and process control, biotechnology and food technology. Welcome are also topics which include chemical aspects of materials, physical chemistry and chemical physics, analytical chemistry, macromolecular chemistry and biomedical engineering.

Stationary phase type and temperature effect on HPLC separation of lactic acid enantiomers

Laura Jánovová, Katarína Hroboňová *

Slovak University of Technology in Bratislava, Faculty of Chemical and Food Technology, Institute of Analytical Chemistry, Radlinského 9, SK-812 37 Bratislava, Slovak Republic

E-mail: *

Abstract: Lactic acid is a biologically important organic acid existing in two enantiomeric forms which are differently metabolized in the human body. In this paper, direct chiral separation of lactic acid by high performance liquid chromatography is presented. Five chiral stationary phases based on macrocyclic antibiotics were used for enantioseparation and chromatographic parameters, such as retention factors, resolution and selectivity factors, were determined under different column temperatures ranging from 5 to 45 °C. Optical isomers of lactic acid were efficiently separated using chiral stationary phases based on teicoplanin (RS = 1.9) and ristocetin (RS = 1.7) in reversed-phase separation mode at the column temperature of 25 °C.

Keywords: chiral separation, high performance liquid chromatography, lactic acid, macrocyclic antibiotics, temperature

Acta Chimica Slovaca, Vol. 14, No. 1, 2021, pp. 60—65, DOI: 10.2478/acs-2021-0008