Spectro-chemometric determination of panthenol enantiomeric excess in pharmaceutical products
EUROFINS BEL/NOVAMANN s.r.o., Komjatická 73, 940 02 Nové Zámky, Slovak Republic
a Polymer institute, Slovak Academy of Sciences, Dúbravská cesta 9, 845 41 Bratislava, Slovak Republic
b Institute of Analytical Chemistry, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37 Bratislava, Slovak Republic
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Abstract: Antibiotic, soothing and healing properties of panthenol are exploited in various pharmaceutical and cosmetic products. Only D-panthenol is biologically active while its L-form might counteract the biological effectiveness of the D-enantiomer. Hydrating and moisturizing effects are exhibited by both enantiomers. Therefore, it is necessary to develop a rapid and cheap method for the determination of panthenol enantiomeric excess in pharmaceutical preparations. In this study, b-cyclodextrin was used as a chiral selector for the recognition of panthenol enantiomers. Inclusion complexes formed by b-cyclodextrin and the analyte showed small differences in NIR and UV/VIS spectra compared by chemometric assessment. Based on the figure of merit and model characteristics, PLS calibration model in the selected range of NIR spectra is preferred. UV/VIS spectrometry has the disadvantage of complicated sample preparation compared to NIR spectrometry. Results reached by both proposed methods were in good agreement. Statistical investigation of the results (by Student t-test and Fisher F-test) confirmed that the proposed methods are comparable and applicable to chiral analysis of panthenol pharmaceutical preparations.
Keywords: chiral analysis, panthenol, pharmaceutical preparations, molecular spectrometry, multivariate calibration
Acta Chimica Slovaca, Vol. 13, No. 2, 2020, pp. 23—29, DOI: 10.2478/acs-2020-0020