Acta Chimica Slovaca (ACS) publishes papers on fundamental and applied aspects of chemistry, biochemistry, chemical technology, chemical engineering and process control, biotechnology and food technology. Welcome are also topics which include chemical aspects of materials, physical chemistry and chemical physics, analytical chemistry, macromolecular chemistry and biomedical engineering.

Spectro-chemometric determination of panthenol enantiomeric excess in pharmaceutical products

Roman Poláček, Angela Kleinová a, Pavel Májek * b

EUROFINS BEL/NOVAMANN s.r.o., Komjatická 73, 940 02 Nové Zámky, Slovak Republic
a Polymer institute, Slovak Academy of Sciences, Dúbravská cesta 9, 845 41 Bratislava, Slovak Republic
b Institute of Analytical Chemistry, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37 Bratislava, Slovak Republic

E-mail: * pavel.majek@stuba.sk

Abstract: Antibiotic, soothing and healing properties of panthenol are exploited in various pharmaceutical and cosmetic products. Only D-panthenol is biologically active while its L-form might counteract the biological effectiveness of the D-enantiomer. Hydrating and moisturizing effects are exhibited by both enantiomers. Therefore, it is necessary to develop a rapid and cheap method for the determination of panthenol enantiomeric excess in pharmaceutical preparations. In this study, b-cyclodextrin was used as a chiral selector for the recognition of panthenol enantiomers. Inclusion complexes formed by b-cyclodextrin and the analyte showed small differences in NIR and UV/VIS spectra compared by chemometric assessment. Based on the figure of merit and model characteristics, PLS calibration model in the selected range of NIR spectra is preferred. UV/VIS spectrometry has the disadvantage of complicated sample preparation compared to NIR spectrometry. Results reached by both proposed methods were in good agreement. Statistical investigation of the results (by Student t-test and Fisher F-test) confirmed that the proposed methods are comparable and applicable to chiral analysis of panthenol pharmaceutical preparations.

Keywords: chiral analysis, panthenol, pharmaceutical preparations, molecular spectrometry, multivariate calibration

Acta Chimica Slovaca, Vol. 13, No. 2, 2020, pp. 1—7, DOI: 10.2478/acs-2020-0020