Acta Chimica Slovaca (ACS) publishes papers on fundamental and applied aspects of chemistry, biochemistry, chemical technology, chemical engineering and process control, biotechnology and food technology. Welcome are also topics which include chemical aspects of materials, physical chemistry and chemical physics, analytical chemistry, macromolecular chemistry and biomedical engineering.

Carbon source utilization and hydrogen production by isolated anaerobic bacteria

R. Jame, V. Zelená, B. Lakatoš, Ľ. Varečka *

Institute of Biochemistry and Microbiology, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, Bratislava 812 37, Slovakia

E-mail: *

Abstract: Five bacterial isolates were tested for their ability to generate hydrogen during anaerobic fermentation with various carbon sources. One isolate from sheep rumen was identified as Escherichia coli and four isolates belonged to Clostridium spp. Glucose, arabinose, ribose, xylose, lactose and cellobiose were used as carbon sources. Results showed that all bacterial strains could utilize these compounds, although the utilization of pentoses diminished growth yield. The excretion of monocarboxylic acids (acetate, propionate, formiate, butyrate) into medium was changed after replacing glucose by other carbon sources. Di- and tricarboxylic acids were excreted in negligible amounts only. Spectra of excreted carboxylic acids were unique for each strain and all carbon sources. All isolates produced Hbetween 4—9 mmol·L–1 during the stationary phase of growth with glucose as energy source. This value was dramatically reduced when pentoses were used as carbon source. Lactose and cellobiose, starch and cellulose were suitable substrates for the Hproduction in some but not all isolates. No Hwas produced by proteinaceous substrate, such as blood. Results show that both substrate utilization and physiological responses (growth, excretion of carboxylates, Hproduction) are unique functions of each isolate.

Keywords: Clostridium sp., glucose, monosaccharides, disaccharides, hydrogen

Full paper in Portable Document Format: acs_0242.pdf

Acta Chimica Slovaca, Vol. 9, No. 1, 2016, pp. 62—67, DOI: 10.1515/acs-2016-0011