Production of galacto-oligosaccharides by commercial preparates of fungal β-galactosidase
Institute of Biotechnology and Food Science, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37 Bratislava, Slovak Republic
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Abstract: Three different fungal β-galactosidases, from three commercial sources, were studied for their capacity to produce galacto-oligosaccharides by trans-galactosylation from lactose. The enzymes were investigated in a soluble form for the GOS production at two concentrations of lactose (10% and 30% w/w). The maximum of GOS concentration (70.9 g. L-1) was obtained at 30% of the initial lactose (pH 4.5, temperature 30 °C) in the second hour of enzyme reaction. The GOS yield (24% w/w) was not significantly affected by the process of immobilisation in comparison with the free enzyme preparation (22% w/w), but time to obtain a maximal GOS concentration was prolonged. Galacto-oligosaccharides were effectively produced by immobilised β-galactosidase in the 15 repeated batch runs of 30% (w/w) of lactose as the substrate.
Keywords: β-galactosidase, galacto-oligosaccharides, immobilisation
Full paper in Portable Document Format: acs_0216.pdf
Acta Chimica Slovaca, Vol. 8, No. 2, 2015, pp. 101—106, DOI: 10.1515/acs-2015-0018