Acta Chimica Slovaca (ACS) publishes papers on fundamental and applied aspects of chemistry, biochemistry, chemical technology, chemical engineering and process control, biotechnology and food technology. Welcome are also topics which include chemical aspects of materials, physical chemistry and chemical physics, analytical chemistry, macromolecular chemistry and biomedical engineering.

Production of galacto-oligosaccharides by commercial preparates of fungal β-galactosidase

Silvia Tokošová, Helena Hronská *, Michal Rosenberg

Institute of Biotechnology and Food Science, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37 Bratislava, Slovak Republic

E-mail: *

Abstract: Three different fungal β-galactosidases, from three commercial sources, were studied for their capacity to produce galacto-oligosaccharides by trans-galactosylation from lactose. The enzymes were investigated in a soluble form for the GOS production at two concentrations of lactose (10% and 30% w/w). The maximum of GOS concentration (70.9 g. L-1) was obtained at 30% of the initial lactose (pH 4.5, temperature 30 °C) in the second hour of enzyme reaction. The GOS yield (24% w/w) was not significantly affected by the process of immobilisation in comparison with the free enzyme preparation (22% w/w), but time to obtain a maximal GOS concentration was prolonged. Galacto-oligosaccharides were effectively produced by immobilised β-galactosidase in the 15 repeated batch runs of 30% (w/w) of lactose as the substrate.

Keywords: β-galactosidase, galacto-oligosaccharides, immobilisation

Full paper in Portable Document Format: acs_0216.pdf

Acta Chimica Slovaca, Vol. 8, No. 2, 2015, pp. 101—106, DOI: 10.1515/acs-2015-0018