Acta Chimica Slovaca (ACS) publishes papers on fundamental and applied aspects of chemistry, biochemistry, chemical technology, chemical engineering and process control, biotechnology and food technology. Welcome are also topics which include chemical aspects of materials, physical chemistry and chemical physics, analytical chemistry, macromolecular chemistry and biomedical engineering.

Screening of carotenoid-producing Rhodotorula strains isolated from natural sources

Jana Tkáčová *, Katarína Furdíková, Tatiana Klempová, Katarína Ďurčanská, Milan Čertík

Department of Biochemical Technology, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Radlinského 9, SK-812 37, Bratislava, Slovak republic

E-mail: *

Abstract: Carotenoids represent large group of various natural pigments ensuring typical coloration of plants, microorganisms and several animals. It was confirmed by many studies, that consuming these biological active compounds has positive impact for human life. Therefore, they are applied in different industrial fields, such as pharmacy, cosmetic, food, and feed industry. Due to high demand for carotenoids we would like to discover new microorganisms overproducing carotenoids. We focused on yeasts of genus Rhodotorula sp. (forty isolates), that we screened according to growth and carotenoid production on Petri dishes and production media. After cultivation on Petri dishes we selected five strains (denoted as KF-4, KF-6, KF-24, KF-31, KF-104) with interesting pigment production and quick growth. The secondary screening on production media identified KF-104 as the best producer of carotenoid pigments with massive pigment accumulation (1.15 mg/g DCW) and yield (9.69 mg/L). The main carotenoid of KF-104 isolate was β-carotene (35.4 %) with the accumulation of 408.7 μg/g DCW and the yield of 3.4 mg/L. The rest were torularhodin, torulene and γ-carotene (62.7–79.0 %). Production of torularhodin in the cells was low (0.4 to 1.4 mg/L) as was its accumulation in cells (31.2–121.0 μg/g DCW). We continue the experimental analyses of these isolates in order understand differences in the content of individual pigments.

Keywords: carotenoids, microbial pigment production, Rhodotorula sp., yeast

Full paper in Portable Document Format: acs_0205.pdf

Acta Chimica Slovaca, Vol. 8, No. 1, 2015, pp. 34—38, DOI: 10.1515/acs-2015-0007