Lectin detection of cell surface saccharides remodeling induced by development of P-glycoprotein mediated multidrug resistance phenotype in L1210 leukemia cells.
Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences, Vlárska 5, 83334 Bratislava, Slovak Republic
a Institute of Biochemistry, Nutrition and Health Protection, Faculty of Chemical and Food Technology, Slovak University of Technology. Bratislava, Radlinského 9, 81237, Slovak Republic.
Abstract: P-glycoprotein is an ATP dependent drug efflux pump the expression of which is responsible for strong depression of cell sensitivities to large group of structurally unrelated substances in neoplastic cells. We found that the expression of this protein in mice leukemia cells L1210 is associated with massive remodeling of cell surface saccharides. This remodeling is consistent with the alteration of cellular contents of UDP-sugars, glycogen and glycoproteins when P-gp positive and P-gp negative L1210 cell variants were compared. The current paper is focused on bringing the state of art information about this topic.
Full paper in Portable Document Format: acs_0186.pdf
Acta Chimica Slovaca, Vol. 7, No. 1, 2014, pp. 52—56, DOI: 10.2478/acs-2014-0010