Acta Chimica Slovaca (ACS) publishes papers on fundamental and applied aspects of chemistry, biochemistry, chemical technology, chemical engineering and process control, biotechnology and food technology. Welcome are also topics which include chemical aspects of materials, physical chemistry and chemical physics, analytical chemistry, macromolecular chemistry and biomedical engineering.

Characterization of vine varieties by SSR markers

Ivana Dokupilová *, Ernest Šturdík a, Daniel Mihálik b

Department of Biotechnology, Faculty of Chemical and Food Technology of Slovak University of Technology, Radlinského 9, 812 37 Bratislava, Slovakia
a Department of Biotechnology, Faculty of Natural Science of University Ss. Cyril and Metodius in Trnava, J. Herdu 2, 917 01 Trnava, Slovakia
b Department of Molecular Biology, The Plant Production Research Centre — Piešťany, Institute of Viticulture and Enology, Bratislavská cesta 122, 92168 Piešťany, Slovakia

E-mail: *

Abstract: Nowadays, there are well known PCR-based techniques to detect polymorphism in plants. Marker technologies which are applied in breeding and varietal characterization can be exchanged across laboratories with standardization to yield reproducible results. This article describes applying of simple sequence repeats (SSR) markers in European laboratories, in which the reproducibility of these popular markers are examined. One of the European Union interests is characterization of grape varieties and preparation of International Vitis catalogue for comparison global varieties as Chardonnay, Sauvignon, Pinot noir with the domestic for each other country. Grape genome contains of many SSR, which are highly polymorphic. This review provides the latest information in SSR research including novels in research, comparison, development and application of microsatellites.

Keywords: Microsatellites — SSR, vine varieties, polymorphism, Vitis vinifera

Full paper in Portable Document Format: acs_0170.pdf

Acta Chimica Slovaca, Vol. 6, No. 2, 2013, pp. 227—234, DOI: 10.2478/acs-2013-0035