Simulating moving bed chromatography separation of galacto-oligosaccharides
Department of Chemical and Biochemical Engineering, Institute of Chemical and Environmental Engineering, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37 Bratislava, Slovakia
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Abstract: A mixture of galacto-oligosaccharides and non-prebiotic sugars was separated using a simulated moving bed unit equipped with eight packed-bed columns of a cation exchanger. In order to determine operating conditions using triangle theory, equilibrium adsorption isotherms of galactose, glucose, lactose, tri- and tetragalacto-oligosaccharide were measured. It was found that each column of the SMB unit had a maximum separation efficiency of more than 3000 theoretical plates. The achieved purity of galacto-oligosaccharides in the raffinate stream was 99.9 %, similarly 99.9 % of extract was formed by non-prebiotic sugars lactose, glucose and galactose. The productivities in the raffinate and extract, were 0.6 g/(L h) and 2.4 g/(L h), respectively.
Keywords: simulated moving bed chromatography, galacto-oligosaccharides, adsorption isotherm
Full paper in Portable Document Format: acs_0168.pdf
Acta Chimica Slovaca, Vol. 6, No. 2, 2013, pp. 206—210, DOI: 10.2478/acs-2013-0033