Acta Chimica Slovaca (ACS) publishes papers on fundamental and applied aspects of chemistry, biochemistry, chemical technology, chemical engineering and process control, biotechnology and food technology. Welcome are also topics which include chemical aspects of materials, physical chemistry and chemical physics, analytical chemistry, macromolecular chemistry and biomedical engineering.

Protective Membranes at Electrochemical Biosensors

Anton Ambrózy *, Lenka Hlavatá, Ján Labuda

Institute of Analytical Chemistry, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Radlinského 9, 812 37 Bratislava, Slovak Republic

E-mail: *

Abstract: The primary role of the biosensor is to specifically detect an analyte using biochemical reaction or interaction mediated by isolated biomolecules, organelles, whole cells or biomimetic receptors. In terms of construction and function, the biosensor consists of biorecognition element and transducer connected to suitable measurement device. Electrochemical biosensor is an electrode or microelectrode with the surface chemically modified by the biorecognition element. The main problem of analysis with the biosensors is the presence of low and high molecular weight substances in the sample that interfere at the detection of analyte. Due to deposition of surface active compounds the biosensor response may be diminished depending on time of interaction with sample. These effects can be eliminated by using anti-interference membranes. This review deals with preparation and utilization of membranes for the biocomponent immobilization and with outer-sphere protective membranes.

Keywords: biosensor, interference, surface active compounds, protective membrane, chemically modified electrode

Full paper in Portable Document Format: acs_0142.pdf

Acta Chimica Slovaca, Vol. 6, No. 1, 2013, pp. 35—41, DOI: 10.2478/acs-2013-0007